For citation:
Sergushichev A. A., Aleksandrov A. V., Kazakov S. V., Tsarev F. N., Shalyto A. A. Combining de Bruijn graphs, overlap graphs and microassembly for de novo genome assembly. Izvestiya of Saratov University. Mathematics. Mechanics. Informatics, 2013, vol. 13, iss. 2, pp. 51-57. DOI: 10.18500/1816-9791-2013-13-2-2-51-57, EDN: RHABJZ
This is an open access article distributed under the terms of Creative Commons Attribution 4.0 International License (CC-BY 4.0).
Published online:
25.05.2013
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Language:
Russian
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UDC:
004.021
EDN:
RHABJZ
Combining de Bruijn graphs, overlap graphs and microassembly for de novo genome assembly
Autors:
Sergushichev Aleksei Aleksandrovich, Saint-Petersburg National Research University of Information Technologies
Aleksandrov Anton Vyacheslavovich, Saint-Petersburg National Research University of Information Technologies
Kazakov Sergei Vladimirovich, Saint-Petersburg National Research University of Information Technologies
Tsarev Fedor Nikolayevich, Saint-Petersburg National Research University of Information Technologies
Shalyto Anatoly Abramovich, Saint-Petersburg National Research University of Information Technologies
Abstract:
In this paper we present a method for de novo genome assembly that splits the process into three stages: quasicontig assembly; contig assembly from quasicontigs; contig postprocessing with microassembly. The first stage uses de Bruijn graph, the second one uses overlap graph. We have carried out experiments of assembling the E. Coli genome (size ≈4.5 Mbp) andMaylandia zebra genome (size ≈1 Gbp). Advantage of proposed method is a low memory consumption.
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Received:
17.11.2012
Accepted:
26.04.2013
Published:
31.05.2013
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